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Determination of ursolic acid content in cosmetics by HPLC

Yuehua Zhang

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Abstract

A HPLC method for the determination of ursolic acid in cosmetics was established.Samples of cosmetics were extracted using methanol as solvent under ultrasonic wave.The extract was analyzed on a Agilent HC-C18 column(250 mm×4.6 mm,5 μm)with UV detection wavelength at 215 nm,the mobile phase consisted of V(CH3OH)∶V(H2O)∶V(CH3COOH)=87∶13∶0.03,at a flow rate of 1.0 mL/min.Results showed that as the concentration of ursolic acid is in the range of 0.2 μg/mL ～103 μg/mL,the relationship between concentration and the corresponding peak area shows good linearity(r0.999 7).The regression equation can be expressed as y=25.367 5x+0.546 9 and the recoveries achieve 96.4%～101.3%.The RSD of the method is below 3.6%.

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What this paper is about

A HPLC method for the determination of ursolic acid in cosmetics was established.Samples of cosmetics were extracted using methanol as solvent under ultrasonic wave.The extract was analyzed on a Agilent HC-C18 column(250 mm×4.6 mm,5 μm)with UV detection wavelength at 215 nm,the mobile phase consisted of V(CH3OH)∶V(H2O)∶V(CH3COOH)=87∶13∶0.03,at a flow rate of 1.0 mL/min.Results showed that as the concentration of ursolic acid is in the range of 0.2 μg/mL ～103 μg/mL,the relationship between concentration and the corresponding peak area shows good linearity(r0.999 7).The regression equation can be expressed as y=25.367 5x+0.546 9 and the recoveries achieve 96.4%～101.3%.The RSD of the method is below 3.6%.

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Available abstract

A HPLC method for the determination of ursolic acid in cosmetics was established.Samples of cosmetics were extracted using methanol as solvent under ultrasonic wave.The extract was analyzed on a Agilent HC-C18 column(250 mm×4.6 mm,5 μm)with UV detection wavelength at 215 nm,the mobile phase consisted of V(CH3OH)∶V(H2O)∶V(CH3COOH)=87∶13∶0.03,at a flow rate of 1.0 mL/min.Results showed that as the concentration of ursolic acid is in the range of 0.2 μg/mL ～103 μg/mL,the relationship between concentration and the corresponding peak area shows good linearity(r0.999 7).The regression equation can be expressed as y=25.367 5x+0.546 9 and the recoveries achieve 96.4%～101.3%.The RSD of the method is below 3.6%.

Key concepts: Ursolic acid, Chromatography, Chemistry, Cosmetics, Solvent, High-performance liquid chromatography, Methanol, Content determination

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