2007•Yunnan Journal of Traditional Chinese Medicine and Materia MedicaRequires access

Determination of Catechin Content in New Antasthmatic Mixture by High-performance Liquid Chromatography

LI Ren-qiu

Abstract

Objective: To establish a high-performance liquid chromatography to determine the catechin content in new antasthmatic mixture.Method: Dikma C18 column(4.6 mm×250 mm,5μm) was used.The mobile phase was acetonitrile-0.2%phosphoric acid solution(8:92).The determination wavelength was 280 mm.The mobile speed was 1.0ml/min.the coloumn temperature was 35oC.Result: The catechin content was within 0.0425-4.25μg(r=0.99999) and had a good linear relation with peak area.The average recoveray rate was 99.05%,RSD=0.72%(n=9).Conclusion: The method is simple,convenient and quick,with a strong exclusive and a good reappearance and can be used in the quality control of the new antasthmatic mixture.

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Key concepts: Catechin, Phosphoric acid, Chromatography, Chemistry, High-performance liquid chromatography, Acetonitrile, Content determination, Analytical Chemistry (journal)