Rapid detemination of amentoflavone in Selagingella by HPLC

Objective To establish a rapid HPLC method for the determination of amentoflavone in 4 herbs of Selagingella Spring.MethodsHPLC analysis was carried out on SHIMADZU VP-ODS(4.6 mm× 250 mm,5 μm) at 30 ℃.The mobile phase was ACN-0.2%HAc.The flow rate was 1.2 mL·min-1.The detection wavelength was 338 nm.ResultsThe linear range of amentoflavone was 0.295～8.853 μg(r= 0.999 99),the average recovery of this method was 99.34%,and the RSD was 0.94%(n=6).The content of amentoflavone in different samples was at 2.176～8.186 mg·g-1.ConclusionThe contents of amentoflavone are obviouslly different in herbs of Selaginella with different localities and species.This quantitative measurement method provides a simple and rapid way for the quality control and species selection.