Tribenzoyl-cellulose as Stationary Phase for Chiral Separation of Optical Isomers

Tribenzoyl-cellulose was synthesized by homogeneous reaction of benzoyl chloride with microcrystalline cellulose in pyridine under ultrasonication at about 45℃.Elemental analysis and IR spectra indicated that almost all hydroxyl groups of the cellulose were converted to ester groups in the pure polymeric form.The prepared tribenzoyl-cellulose was used as chiral stationary phase of thin-layer chromatography to separate three chiral molecules,2-(9-anththryl)-2-methoxyacetic acid,2-(9-anthryl)-2-hydroxyacetic acid and 2-amino-3-phenylpropanoic acid.The three chiral molecules were completely separated by using TEAA,acetonitrile-1%TEAA(2∶1) and ethyl acetate-H2O-acetic acid(6∶2∶1) as developer,respectively.