Chiral Separation of Optical Isomers with Cellulose Tris(benzoate)s as Stationary Phase

Three cellulose derivatives,viz.cellulose tribenzoyl(CTB),cellulose tris(p-chloro-benzoate)(CTPCB) and cellulose tris(o-chlorobenzoate)(CTOCB) were ultrasonically prepared and characterized by IR spectrometry and elemental analysis.They were used as chiral stationary phase of thin-layer chromatography to separate five chiral molecules,viz.2-(9-anththryl)-2-methoxyacetic acid,2-(9-anthryl)-2-hydroxyacetic acid,2-amino-3-phenylpropanoic acid,histidine and chlorpheniramine.The five chiral molecules were completely separated with their respective developing solvents,viz.TEAA,acetonitrile-TEAA(2 ∶1,by volume),ethyl acetate-H2O-acetic acid(6 ∶2 ∶1,by volume),n-butyl alcohol-acetic acid-H2O(4 ∶1 ∶1,by volume) and ethyl acetate-methyl alcohol-30%acetic acid(5 ∶3 ∶2,by volume).The three chiral stationary phases were compared and the results showed that CTB was the best stationary phase for the chiral separation.