Evaluation and Application of Cellulose Triphenylcarbamate Chiral Column

Cellulose triphenylcarbamate (CTPC) were synthesized by the reaction of micrystalline cellulose with phenyliscocyanate in dry pyridine at 110 ℃. Elemental analysis and IR spectra indicated that most of the hydroxy groups of the microcrystalline cellulose had been converted into ester groups .The chiral stationary phases(CSP)were prepared by coating cellulose triphenylcarbamate on the aminopyopyl silica. The evaluation of column was achieved and 12 chiral substances were separated using hexane/2-propanol (90∶10 volume ratio) as mobile phase at a flow rate of 1mL·min~(-1) (at 25 ℃). And the results of the separation of chiral compound showed that the chiral stationary had good steroelectivity.