Study on the Mycelium Submerged Culture of Dictyophora echinovolvata

Physical and chemical factors for the mycelium submerged culture of Dictyophora echinovolvata were studied. The physical and chemical factors are carbon,nitrogen,inorganic salt,vatamin,pH,temperature,quantity of inoculation,quantity of ventilation and time of culture. With the growth of mycelium the change of recursive sugar ,total sugar,pH in culture medium were also studied. Experimental results showed that the composition of fermentation medium as follow: 250 ml malty juice (ρ=1 05 g/cm 3),10 g soluble starch,15 g bean powder,5 g yeast powder,3 g KH 2PO 4,0 5 g MgSO 4 ,0 5 g ZnSO 4,0 5 g MnSO 4,0 1 g VB 1,0 1 g VB 2 and 750 ml H 2O The physical factors as follow: T27°C,pH5 0～6 0,50 ml culture medium/250 ml cone flask,6% inoculated quantity and culture 4 days were the best adoption. The dry weight of mycelium increased by 80 25%.