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2002•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access

Study on the conditions of submerged culture of Grifola frondosa

Limin Guo

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Abstract

By submerged culture of Grifola frondosa,the results showed the optimum carbon source was corn powder + bran,the optimum nitrogen source was peptone and beef cream and the optimum inorganic salt was the combination of MgSO 4,KH 2PO 4,FeSO 4,ZnSO 4,CuSO 4 in liquid culture medium,which was beneficial to mycelium growing.By orthogonal experiments and the research on the influencing factors of biomass of mycelium,the best technical condition of submerged culture was gained.In addition,at pH 5.50 -6.50 and the volume ratio of flow of air-compress at 1.00-1.40,the biomass of mycelium reached the highest level.

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What this paper is about

By submerged culture of Grifola frondosa,the results showed the optimum carbon source was corn powder + bran,the optimum nitrogen source was peptone and beef cream and the optimum inorganic salt was the combination of MgSO 4,KH 2PO 4,FeSO 4,ZnSO 4,CuSO 4 in liquid culture medium,which was beneficial to mycelium growing.By orthogonal experiments and the research on the influencing factors of biomass of mycelium,the best technical condition of submerged culture was gained.In addition,at pH 5.50 -6.50 and the volume ratio of flow of air-compress at 1.00-1.40,the biomass of mycelium reached the highest level.

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Available abstract

By submerged culture of Grifola frondosa,the results showed the optimum carbon source was corn powder + bran,the optimum nitrogen source was peptone and beef cream and the optimum inorganic salt was the combination of MgSO 4,KH 2PO 4,FeSO 4,ZnSO 4,CuSO 4 in liquid culture medium,which was beneficial to mycelium growing.By orthogonal experiments and the research on the influencing factors of biomass of mycelium,the best technical condition of submerged culture was gained.In addition,at pH 5.50 -6.50 and the volume ratio of flow of air-compress at 1.00-1.40,the biomass of mycelium reached the highest level.

Key concepts: Grifola frondosa, Mycelium, Bran, Biomass (ecology), Liquid culture, Chemistry, Pulp and paper industry, Nitrogen

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