Study on the Conditions of Liquid Culture of Inonotus obliquus
Zhou Li-jie
Abstract
Zhou Li-jie
Abstract
The effects of different carbon sources,nitrogen sources,agar,natural substance,culture time and volume of culture solution on liquid culture of Inonotus obliquus were studied.The results indicated that the most suitable culture medium was glucose 20.0 g/L,starch 50.0 g/L,peptone 3.5 g/L,KH_2PO_4 3.0 g/L and MgSO_4 1.5 g/L.The biomass of dry mycelium was 0.181 2 g/100mL.When the culture medium was optimum,culture time was 5 days,and the volume of culture solution was 1/3,the biomass of mycelium reached the highest,0.041 4 g/50mL
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The effects of different carbon sources,nitrogen sources,agar,natural substance,culture time and volume of culture solution on liquid culture of Inonotus obliquus were studied.The results indicated that the most suitable culture medium was glucose 20.0 g/L,starch 50.0 g/L,peptone 3.5 g/L,KH_2PO_4 3.0 g/L and MgSO_4 1.5 g/L.The biomass of dry mycelium was 0.181 2 g/100mL.When the culture medium was optimum,culture time was 5 days,and the volume of culture solution was 1/3,the biomass of mycelium reached the highest,0.041 4 g/50mL
Key concepts: Inonotus obliquus, Mycelium, Liquid culture, Agar, Biomass (ecology), Volume (thermodynamics), Starch, Chemistry