An experimental study on the change of expression of CD44 in rats tissues of lungs in severe acute pancreatitis and the protective function of Qing Yi II

Objective To observe the CD44 expression in the pancreas and lungs and explore the mechanism of Chinese medicine Qing Yi II on treating rats followed sodium taurocholate-induced severe acute pancreastitis(SAP).Methods 36 healthy SD rats were randomly divided into three groups:the sham-operated group(Group A),SAP model group(Group B)and the treated group(Group C),Rats in Group B and C were into SAP model by retrograde injection of 30 g/L sodium taurocholate into bilio-pancreatic duct.Qing Yi II Granules was administered,beginning from 1 hour after modeling for three times(every 6 hours)per day via intragastric in fusion to roupC in dose of 10 ml/kg(250 g/L).While to the other two groups,equal volume of saline was infused instead.All animals were sacrificed after modeling.The pancreas and lungs were teken and the CD44 expression of tissue were examined.Pathologic change of pancreas、lungs tissues were observed under microscope and examined the lung wet/dry weight.Results The expression of tissue CD44 were higher in Group B than that in Group A(P0.01),and lower in Group C than that in group B(P0.05),Group B had serious pathologic damage in pancreas and lungs.Conclusions The expression of CD44 might play an important role in organ dysfunction of the pancreas and lungs in SAP rats.Chinese medicine Qing Yi II Granules could depress the expression of tissue CD44 and alleviate the pathologic damage of the pancreas and lungs in rats with acute necrotizing pancreatitis-associated organ injury.