Application of Pathogen Isolation and PCR Assay for Rapid Detection of Streptococcus Suis Serotype2 from Human Case

Objective To isolate Streptococcus suis strains and to study the biological characteristics,a highly specific,sensitive and rapid method for the detection of streptococcus suis by PCR was developed.Methods Blood-agar substrates were used to cultivate and isolate pathogens from the blood(anticoagulation)of suspect patients.The morphology of the isolated bacteria was observed after staining and the specific serotypes were identified by API 32 Strep biochemical tests.The blood sample(anticoagulation)was taken from the suspect patients,DNA was extracted as template and the primer was synthesized according to the gene sequence to detect 16SrRNA,capsule polysaccharide 2J(cps2J),muramidase-released protein(mrp)and extracellular factor protein(EF).Results Streptococcus suis serotype 2 was founded in the specimens from the suspect patients by PCR and was further confirmed by API 32 Strep biochemical test.Conclusions Result of molecular biology is identical with etiology.The results suggest that PCR assay is a highly specific,sensitive and rapid method for the detection of serotype of Streptococcus suis.