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Establishment of Detection of Streptococcus Suis Type 2 Using Rapid PCR

Zhu Lin-yang

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Abstract

[Objective] To develop a rapid,specific and sensitive method for the detection of streptococcus suis.[Methods] DNA was extracted from the blood(anticoagulation) of patients with suspected Streptococcus suis infection as template,and then synthesized the primers to detect 16 Sr RNA,cps2J,mrp,ef.[Results] PCR Amplification results confirmed that the suspected patient was infected with streptococcus suis II.[Conclusion] PCR Reaction System is a rapid,specific and sensitive method for the detection of serotype of streptococcus suis.

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What this paper is about

[Objective] To develop a rapid,specific and sensitive method for the detection of streptococcus suis.[Methods] DNA was extracted from the blood(anticoagulation) of patients with suspected Streptococcus suis infection as template,and then synthesized the primers to detect 16 Sr RNA,cps2J,mrp,ef.[Results] PCR Amplification results confirmed that the suspected patient was infected with streptococcus suis II.[Conclusion] PCR Reaction System is a rapid,specific and sensitive method for the detection of serotype of streptococcus suis.

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Available abstract

[Objective] To develop a rapid,specific and sensitive method for the detection of streptococcus suis.[Methods] DNA was extracted from the blood(anticoagulation) of patients with suspected Streptococcus suis infection as template,and then synthesized the primers to detect 16 Sr RNA,cps2J,mrp,ef.[Results] PCR Amplification results confirmed that the suspected patient was infected with streptococcus suis II.[Conclusion] PCR Reaction System is a rapid,specific and sensitive method for the detection of serotype of streptococcus suis.

Key concepts: Streptococcus suis, Serotype, Microbiology, Streptococcus, Polymerase chain reaction, Virology, Real-time polymerase chain reaction, Biology

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