2012•Journal of Guangdong Pharmaceutical UniversityRequires access

Study on the determination of total flavonoids in Kangli capsule by ultraviolet spectrophotometry

Cui Sheng-miao

Abstract

Objective To establish a method for the determination of total flavonoids content in Kangli capsule.Methods The detection was set at 504 nm with rutin as reference by ultraviolet spectrophoto-metry.The content of the total flavonoids was employed as the index to investigate the adsorption and elution of them on polyamide.Results The method was linear within the range of 6.891-34.456 μg/mL.The average recovery of the assay was 102.4% and RSD was 1.9%.Conclusion The method was simple,highly accurate and nontoxic,which may be used in the assay of total flavonoids in Kangli capsule.

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Key concepts: Capsule, Rutin, Chromatography, Chemistry, Ultraviolet, Polyamide, Content determination, Elution