2014•Unpublished venueRequires access

Determining the Content of Total Flavonoids in Cuiqingzhuyun Solution with Ultraviolet Spectrometry

Wang Dong-shen

Abstract

To determine the content of total flavonoids in Cuiqingzhuyun solution,icariin was used as standard solution and methanol was used as blank solution to measure the absorbance of the sample solution at 270 nm with UV spectophotometry.The results showed that the linear range for the content of icariin was 1.68~10.08 μg / mL with the correlation coefficient of0.999 5. The average recovery of total flavonoids was 99.29% and RSD was 1.20%. The content of total flavonids of Cuiqingzhuyun solution was 1.317 9%. It is indicated that method of determining of total flavonoids in Cuiqingzhuyun solution with UV spectrophotometry was rapid, accurate, convenient and practical.

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Key concepts: Chemistry, Absorbance, Icariin, Chromatography, Correlation coefficient, Content determination, Spectrophotometry, Content (measure theory)