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2010•Ningxia Medical JournalRequires access

Determination of Total flavonoids in Yishoudixian Granule

Zhang Dong-ping

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Abstract

Objective To establish a method for the determination of total flavonoids in Yishoudixian Granule by ultraviolet spectrophotometry.Methods Determination of flavonoids was adopted by ultraviolet spectrophotometry in Yishoudixian Granule of content.Results It was apparent total flavonoids in the 0.0052 mg/ml-0.0416 mg/ml and a good linear relationship between the absorbance.For the regression equation y = 12.15x +0.0035 r2 = 0.995,flavonoids in total was 5.86 %.The sample was stable and reproducible within 100 min,and RSD was 1.24 %,and the average recovery rate was 95.51 %.Accuracy of the relative deviation was 0.09396%.Conclusion This method is simple,reliable a method for determination of Yishoudixian granule particles.

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What this paper is about

Objective To establish a method for the determination of total flavonoids in Yishoudixian Granule by ultraviolet spectrophotometry.Methods Determination of flavonoids was adopted by ultraviolet spectrophotometry in Yishoudixian Granule of content.Results It was apparent total flavonoids in the 0.0052 mg/ml-0.0416 mg/ml and a good linear relationship between the absorbance.For the regression equation y = 12.15x +0.0035 r2 = 0.995,flavonoids in total was 5.86 %.The sample was stable and reproducible within 100 min,and RSD was 1.24 %,and the average recovery rate was 95.51 %.Accuracy of the relative deviation was 0.09396%.Conclusion This method is simple,reliable a method for determination of Yishoudixian granule particles.

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Available abstract

Objective To establish a method for the determination of total flavonoids in Yishoudixian Granule by ultraviolet spectrophotometry.Methods Determination of flavonoids was adopted by ultraviolet spectrophotometry in Yishoudixian Granule of content.Results It was apparent total flavonoids in the 0.0052 mg/ml-0.0416 mg/ml and a good linear relationship between the absorbance.For the regression equation y = 12.15x +0.0035 r2 = 0.995,flavonoids in total was 5.86 %.The sample was stable and reproducible within 100 min,and RSD was 1.24 %,and the average recovery rate was 95.51 %.Accuracy of the relative deviation was 0.09396%.Conclusion This method is simple,reliable a method for determination of Yishoudixian granule particles.

Key concepts: Granule (geology), Absorbance, Spectrophotometry, Chromatography, Linear relationship, Relative standard deviation, Recovery rate, Medicine

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