Determination of total flavonoids in compound hongjingtian capsule by UV-visible spectrophotometry

Objective To establish UV-visible spectrophotometry method for the determination of the total flavonoids in compound Hongjingtian capsule.Methods The contents of the total flavonoids were determined at 508 nm with rutin as the standard substance by ultraviolet UV-visible spectrophotometry method.Results The calibration curve of rutin was linear when its concentration range was from 8.0 to 48.0mg/l(r=0.9998),and the average recoveries was 99.83%.The content of total flavonoids in 3 batches of sample was 1.10%,1.08%,1.12%.Conclusion The method is simple,high sensitivity and reliable in results,and can be used as the determination of the total flavonoids in compound Hongjingtian capsule.