Effects of 6-BA and NAA on Different Explants of Cultured Tissues of Alfalfa

The paper studied effects of seven kinds of medium on induction and growth of callus,using different of explant of Medicago varia.cv.Xinmu No.1 and Medicago sativa L.cv.Xinjiang Daye,and effects of different concentration of 6-BA(6-Benzylaminopurine) and NAA(1-Naphthyacetic Acid) on induction,differentiation callus.The results are as follows:(1) The concentration of 2～8 mg/L,NAA is benefit to induce callus,if it's concentration was higher,the callus of alfalfa were better.The concentration of 1～3 mg/L,1mg/L 6-BA is benefit toinduce callus,if it's concentration was higher,the callus of alfalfa were worse.(2)MS+2,4-D 2 mg/L +NAA 4 mg/L +6-BA 1 mg/L was the better induction medium of Xinmu No.1.MS+2,4-D 2 mg/L +NAA 8 mg/L +6-BA 1 mg/L was the better induction medium of Xinjiang Daye.(3)Using the explant of hypocotyls,cotyledon and leaves as explants,hypocotyls was the best explant of induction callus medium.