Study on Callus Induction and Subculture of Leaf Culture of Tobacco Variety-K326

The leaf of tobacco variety-K326 was used as the explants on the MS basal medium and the influence on callus induction by different hormones,as well as the influence on its subculture with the combination of NAA and 6-BA were studied.The results showed that 2.4-D 2.0 mg/L + 6-BA 0.5 mg/L was the most effective combination for callus induction.During the subculture,the different concentration of NAA + 6-BA combination had different effect on callus growth,structure,texture,color and luster,and the combinations of NAA 0.1 mg/L + 6-BA 3.0 mg/L,NAA 2.0 mg/L + 6-BA 0.5 mg/L and NAA 1.5 mg/L + 6-BA 0.5 mg/L were preferable.Callus on the medium with NAA 0.1 mg/L + 6-BA 3.0 mg/L were the green color with yellow,tightness and hard.Callus on NAA 2.0 mg/L + 6-BA 0.5 mg/L and NAA 1.5 mg/L + 6-BA 0.5 mg/L were the yellow color with white,loose and soft.