Effects of different plant growth regulators on callus induction from leaf explant of Camellia granthamina

The young leaf of Camellia granthamina were used as explants in the tissue culture research.The effects of different combination of 6-BA,TDZ,KT,2,4-D and NAA on callus induction were investigated.The results indicated that the appropriate medium for callus induction was MS+2.5 mg/L 6-BA+0.5 mg/L NAA;MS+6-BA 2.5 mg/L + NAA 0.5 mg/L medium respectively supplied with 2,4-D 0.5~2.5 mg/L,KT 0.5～2.5 mg/L,TDZ 0.1～0.5 mg/L,2,4-D and TDZ improved callus induction rate up to 100% and 94.8%,KT reduced callus induction;the appropriate medium for differentiation was MS+ 2.5 mg/L 6-BA + 0.02 mg/L TDZ + 0.05 mg/L NAA.