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2006•Unpublished venueRequires access

Determination of puerarin in Yufengningxin soft-gel capsules by HPLC

HE Zhong-gui

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Abstract

Objective To establish an HPLC method for the determination of puerarin in Yufengningxin soft-gel capsules.Methods The sample was analyzed on a Diamonsil C_(18) column(200 mm×4.6 mm,5 μm).A mixture of methanol-0.1 mol/L sodium acetate(25∶75,adjusted to pH 5.0 with acetic acid) was used as the mobile phase with a flow rate of 1.0 mL/min.The detection wavelength was 250 nm.Results The linear range of puerarin was 0.2006~1.0030 μg(r=0.999 9).The average recovery of puerarin was 99.93% with RSD 0.79%.Conclusions The method is accurate and can be used for the quality control of Yufengningxin soft-gel capsules.

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What this paper is about

Objective To establish an HPLC method for the determination of puerarin in Yufengningxin soft-gel capsules.Methods The sample was analyzed on a Diamonsil C_(18) column(200 mm×4.6 mm,5 μm).A mixture of methanol-0.1 mol/L sodium acetate(25∶75,adjusted to pH 5.0 with acetic acid) was used as the mobile phase with a flow rate of 1.0 mL/min.The detection wavelength was 250 nm.Results The linear range of puerarin was 0.2006~1.0030 μg(r=0.999 9).The average recovery of puerarin was 99.93% with RSD 0.79%.Conclusions The method is accurate and can be used for the quality control of Yufengningxin soft-gel capsules.

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Available abstract

Objective To establish an HPLC method for the determination of puerarin in Yufengningxin soft-gel capsules.Methods The sample was analyzed on a Diamonsil C_(18) column(200 mm×4.6 mm,5 μm).A mixture of methanol-0.1 mol/L sodium acetate(25∶75,adjusted to pH 5.0 with acetic acid) was used as the mobile phase with a flow rate of 1.0 mL/min.The detection wavelength was 250 nm.Results The linear range of puerarin was 0.2006~1.0030 μg(r=0.999 9).The average recovery of puerarin was 99.93% with RSD 0.79%.Conclusions The method is accurate and can be used for the quality control of Yufengningxin soft-gel capsules.

Key concepts: Puerarin, Chromatography, Chemistry, High-performance liquid chromatography, Acetic acid, Sodium acetate, Linear range, Detection limit

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