Effects of Mesenchymal Stem Cell Line on Differentiation and Maturation of Murine Bone Marrow-derived Dendritic Cells

Objective To study on the biological characteristics of murine bone marrow-derived dendritic cells(DCs)regulated by mesenchymal stem cell line C3H10T1/2 in vitro.Methods Murine bone marrow cells were isolated by density gradient centrifugation and cultured with mGM-CSF and mIL-4.Large amounts of immature DCs(iDCs)were obtained.Then DCs were co-cultured with C3H10T1/2 cells in the presence of LPS for 2 days.The effects of C3H10T1/2 cells on the maturation of DCs were analyzed with inverted microscope,flow cytometry,mixed lymphocyte reaction and ELISA.Results DCs co-cultured with C3H10T1/2 cells were scattered and round observed under inverted microscope.FACS analyses showed that those DCs expressed low levels of CD11c,MHC-Ⅱ,CD80,CD86 and CD40.Allogeneic spleenocytes proliferation assay showed that the stimulatory ability of DCs from co-culture group was significantly lower than that in the control group.The IFN-γ and IL-10 secretion in the culture supernants were lower in the co-culture group compared with that in the control group.Conclusion Mesenchymal stem cell line C3H10T1/2 can significantly suppress the maturation of murine bone marrow derived DCs in vitro.