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Isolation,Culture and Identification of Rat Bone Marrow-derived Dendritic Cells

Shengning Zhang

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Abstract

Objective To explore methods for isolating,culturing and identificating of rat bone marrow-derived dendritic cells.Methods DCs obtained from wistar rat bone marrow were cultured in vitro under the condition of GM-CSF and IL-4.The morphology,phenotype,and function of DCs were identified.ResuIts Mature rat bone marrow-derived DCs were obtained 9 d after cultured in vitro.More than 90% of cultured DCs expressed OX62(the marker of rat DCs),and most mature DCs expressed major histocompatibility complex(MHC)class II,CD80 and CD86 with high level.Mature DCs stimulated effectively MLR.ConcIusion A method to generate large number of DCs from rat bone marrow in vitro has been established.

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What this paper is about

Objective To explore methods for isolating,culturing and identificating of rat bone marrow-derived dendritic cells.Methods DCs obtained from wistar rat bone marrow were cultured in vitro under the condition of GM-CSF and IL-4.The morphology,phenotype,and function of DCs were identified.ResuIts Mature rat bone marrow-derived DCs were obtained 9 d after cultured in vitro.More than 90% of cultured DCs expressed OX62(the marker of rat DCs),and most mature DCs expressed major histocompatibility complex(MHC)class II,CD80 and CD86 with high level.Mature DCs stimulated effectively MLR.ConcIusion A method to generate large number of DCs from rat bone marrow in vitro has been established.

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Available abstract

Objective To explore methods for isolating,culturing and identificating of rat bone marrow-derived dendritic cells.Methods DCs obtained from wistar rat bone marrow were cultured in vitro under the condition of GM-CSF and IL-4.The morphology,phenotype,and function of DCs were identified.ResuIts Mature rat bone marrow-derived DCs were obtained 9 d after cultured in vitro.More than 90% of cultured DCs expressed OX62(the marker of rat DCs),and most mature DCs expressed major histocompatibility complex(MHC)class II,CD80 and CD86 with high level.Mature DCs stimulated effectively MLR.ConcIusion A method to generate large number of DCs from rat bone marrow in vitro has been established.

Key concepts: Bone marrow, Follicular dendritic cells, In vitro, Major histocompatibility complex, CD80, CD86, Cell biology, Biology

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