Activating effect of dendritic cells derived from murine bone marrow on T cell activity in mice

Objective To induce and proliferate dendritic cells (DCs) derived from murine bone marrow and to detect the proliferation activity of allogenic T cells stimulated by DCs in vitro. MethodsThe mononuclear cells (MNCs) isolated from murine bone marrow were cultured in RPMI medium 1640 containing reconstructive mouse granulocyte macrophage colony-stimulating factor (rmGM-CSF),interleukin-4 (IL-4), and tumor necrosis factor-α(TNF-α) to generate DCs. The morphologic characteristics of the cells were observed with light microscopes and the phenotypic figures were analysed with FACS. The proliferation activity of allogenic T cells stimulated by DCs was detected by MLR. Results The MNCs were cultured in present of rmGM-CSF, IL-4, and TNF-α for 12 days, these cells bear typical morphologic and phenotypic characteristics of dendritic cells, expressed MHCⅡ, CD11c, CD80, and CD86 antigen highly, gained the powerful capacity to stimulate allogeneic T cells. Conclusion Induced proliferated DCs strongly activate the activity of allogenic T cells.