Preparation and Identification of Dendritic Cells Derived from Bone Marrow Cells of BALB/c Mouse

Objective To study the preparation and identification methods of dendritic cells(DCs)from bone marrow cells of mice,and supply reference for the cell preparation and in vitro research.Methods The bone marrow cells from BALB/c mice were treated with GM-CSF,IL-4 and LPS to prepare matured DCs.Optical microscopy and scanning electron microscopy were applied to observe the morphological character of the prepared cells.The markers of DCs on the cells' surface and some cytokines secreted by the cells were detected.Results Typical morphological characters were observed in the cells.The expressions of CD1 a,CD11c,CD80,and CD86 in the cells and also the secretion of TNF-α,IFN-γwere increased significantly.Conclusion The treatment with GM-CSF,IL-4,LPS to BALB/c mouse bone marrow cells can induce high production of mature DCs in vitro.