2011•Zhonghua zhongyiyao zazhiRequires access
Effect of Bushen Kangshuai Tablets on markers indicative of oxidative stress in rabbits hippocampus
Zhou Ya-nan
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Abstract
Objective:To investigate the effect of Bushen Kangshuai Tablets in treating atherosclerotic lesion and the mechanism of oxidative stress in rabbits hippocampus.Methods:The 56 Japanese white rabbits were divided into normal group 8,and the experimental group 48 randomly,the normal group were given normal diet,the experimental group a reference to the establishment of experimental atherosclerosis model of oxidative stress in the first 8 weeks.Experimental group randomly divided into model group,Bushen Kangshuai group and simvastatin group.Each group were sacrificed after drug administration for 16 weeks after the brain was removed under sterile conditions,using Real Time Quantitative PCR check the expression of HO-1mRNA of hippocampus,SOD,GSH-Px and MDA concentrations in serum were detected by Enzyme-linked immunosorbent assay(ELISA).Results:HO-1 mRNA gene expressed in rabbit hippocampus in each group,there was no significant difference in the expression of HO-1 mRNA gene between the normal and the model group,but after treated with Bushen Kangshuai and simvastatin tablets,the mRNA level of HO-1 increased significantly(P0.05);SOD,MDA and GSH-Px were significantly higher than the normal group(P0.05 or P0.01).Conclusion:Chinese herbal compound Bushen Kangshuai Tablets in atherosclerosis induced brain injury had an important antioxidant role,the way may be through regulatied the HO-1mRNA gene expression,or may be stimulated the up regulation of SOD,GSH-Px and scavenged the MDA.