Study on the changes of NO,SOD and MDA in serum of osteoporosis in ovariectomied rats and the effects of formula granule of Jiawei Bushen Zhuangjin decoction on its

Objective:To explore the changes of NO,SOD,MDA in serum of osteoporosis in ovariectomied rats and the effects of granule of Jiawei Bushen Zhuangjin decoction on its.Methods:Forty-five female Wistar rats aged 6 months were divided into three groups randomly:control group,model group and herb granular group.Excising the ovaries of rats formed the animal's model of osteoporosis in model herb granular groups.Sodium chloride of 0.9% was given to stomachs of rats in control and model groups with 6.7 ml·kg~-1 ·d~-1 ,herb granular was given to stomachs of rats in herb granular group with ~6.7 ml·kg~-1 ·d~-1 . The levels of NO,SOD and MDA in serum were measured at pre-immobilization and 3 months after immobilization.Results:3 months after immobilization,there were much lower NO,SOD levels in model group than those in control group,as well as much higher MDA levels in model group than those in control group(P0.05).Compared with model group,the levels of NO and SOD in serum evidently raised and the MDA decreased after using granular of Jiawei Bushen Zhuangjin decoction(P0.05).Conclusion:NO,SOD and MDA in serum may be participate in the pathological process of postmenopausal osteoporosis.Granular of Jiawei Bushen Zhuangjin decoction can adjust the levels of NO,SOD and MDA in serum,which may be the mechanism of the drugs for preventing and treating osteoporosis.