Simultaneous determination of five sesquiterpene content in Curcumae Rhizoma from different areas by HPLC

OBJECTIVE To establish an HPLC method for the simultaneous determination of five sesquiterpene content of curdione,curcumol,germacrone,furanodiene and β-elemene in Curcumae Rhizoma from different areas.METHODS The separation was performed on a Kromasil C18 column(4.6 mm × 250 mm,5 μm) with the mobile phase consisting of acetonitrile(A)-water(B) by gradient elution.The gradient condition was as follows: 0-35 min,45% A→70% A;35-40 min,70% A→90% A;40-60 min,90% A→95% A.The flow rate was 1.0 mL· min-1.The column temperature was set at 25 ℃ and the UV detection wavelength was 214 nm.RESULTS The linear ranges and correlation coefficients of curdione,curcumol,germacrone,furanodiene and β-elemene were 27.88-278.8,20.42-204.2,3.475-34.75,5.380-53.80,10.12-101.2 μg·mL-1 and r≥0.999 6.The average recoveries were 97.36%,97.96%,97.47%,98.70% and 99.05% with corresponding RSD of 1.85%,2.30%,2.02%,2.40% and 1.91%.CONCLUSION The method is simple,accurate,reproducibility and can be used to determine curdione,curcumol,germacrone,furanodiene and β-elemene in Curcumae Rhizoma from different areas.