Content Determination of Hydroxyisogermafurenolide in Curcumae Rhizoma from Different Habitats by HPLC

OBJECTIVE:To establish the method for the content determination of hydroxyisogermafurenolide in Curcumae Rhizoma,and to compare the content difference of hydroxyisogermafurenolide in Curcumae Rhizoma from different habitats. METHODS:HPLC method was adopted. The separation was performed on Kromasil C18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of acetonitrile-water(gradient elution)at the flow rate of 1.0 ml/min. The detection wavelength was set at 214 nm and column temperature was 25 ℃. RESULTS:The linear range of hydroxyisogermafurenolide were 1.47-14.68 μg/m(lr=0.999 7).RSDs of precision,stability and reproducibility tests were all lower than 3%. The average recovery were 95.31%-95.80%(RSD were 0.07%-1.36%,n=3). The contents of hydroxyisogermafurenolide in same types of Curcumae Rhizoma from different habitats were different from that of Curcumae Rhizoma from same habitats at different periods. CONCLUSIONS:The method is well-separated,specific,reproducible,simple and feasible. It can be used for the content determination of hydroxyisogermafurenolide in Curcumae Rhizoma and improve the present quality standard of Curcumae Rhizoma.