Simultaneous Determination of Curdione,Curcumol,Germacrone,Curzerene,Furanodiene,and β-Elemene in Curcuma zedoary by RP-HPLC

Objective: To establish the method for determining curdione,curcumol,germacrone,curzerene,furanodiene and β-elemene in Curcuma zedoary by RP-HPLC.Method: A Hypersil ODS column(4.6mm×250 mm,5 μm) was used with the mixture of acetonitrile-0.1% phosphoric acid as the mobile phase in gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was kept at 25 ℃.The detection wavelength was set at 215 nm.Result: The calibration curves of curdione,curcumol,germacrone,curzerene,furanodiene and β-elemene were in good linearity over the ranges of 0.544-5.44 μg(r = 0.999 6),0.414-4.14 μg(r =0.999 8),0.122-1.22 μg(r =0.999 5),1.78-17.8 μg(r =0.999 3),0.318-3.18 μg(r =0.999 4),0.506-5.06 μg(r = 0.999 5) respectively,and the average recoveries were 99.60%(RSD 2.71%),101.48%(RSD1.37%),99.50%(RSD 2.47%),100.29%(RSD 2.52%),99.87%(RSD 1.51%),100.58%(RSD1.33%) respectively.Conclusion: The method is sensitive,accurate; the separation effect is good without interference.It can provide a basis as evaluation standard for quality of Rhizoma Zedoariae,and provide scientific basis for clinical rational drug use.