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Determination of Curdione,Curcumol and Germacrone in Rhizoma Curcumae by HPLC

Jiang Guo-fei

Open publisher page 1 citations

Abstract

OBJECTIVE To establish a high performance liquid chromatography method for the determination of three components in herbal pieces of Rhizoma Curcuma prepared for decoction.METHODS Elite Hypersil ODS column(4.6 mm×250 mm,5 μm) and acetonitrile-water were the stationary and the mobile phases respectively.Flow rate and the column temperature were 1 mL/min and 25 ℃.The detection wavelength was 216 nm and the injection volume was 10 μL.RESULTS The calibration curve for curdione,curcumol and germacrone revealed linearity in the range of 0.008 1~0.257 4,0.006 7~0.213 6,0.001 6~0.050 0 mg/mL,respectively.The average recovery rate was 99.1%,98.6% and 98.2%,with RSD of 2.3%,1.4% and 2.0%.CONCLUSION The method is reliable,simple and accurate,which can be used to identify and evaluate the quality of Rhizoma Curcumae.

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What this paper is about

OBJECTIVE To establish a high performance liquid chromatography method for the determination of three components in herbal pieces of Rhizoma Curcuma prepared for decoction.METHODS Elite Hypersil ODS column(4.6 mm×250 mm,5 μm) and acetonitrile-water were the stationary and the mobile phases respectively.Flow rate and the column temperature were 1 mL/min and 25 ℃.The detection wavelength was 216 nm and the injection volume was 10 μL.RESULTS The calibration curve for curdione,curcumol and germacrone revealed linearity in the range of 0.008 1~0.257 4,0.006 7~0.213 6,0.001 6~0.050 0 mg/mL,respectively.The average recovery rate was 99.1%,98.6% and 98.2%,with RSD of 2.3%,1.4% and 2.0%.CONCLUSION The method is reliable,simple and accurate,which can be used to identify and evaluate the quality of Rhizoma Curcumae.

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Available abstract

OBJECTIVE To establish a high performance liquid chromatography method for the determination of three components in herbal pieces of Rhizoma Curcuma prepared for decoction.METHODS Elite Hypersil ODS column(4.6 mm×250 mm,5 μm) and acetonitrile-water were the stationary and the mobile phases respectively.Flow rate and the column temperature were 1 mL/min and 25 ℃.The detection wavelength was 216 nm and the injection volume was 10 μL.RESULTS The calibration curve for curdione,curcumol and germacrone revealed linearity in the range of 0.008 1~0.257 4,0.006 7~0.213 6,0.001 6~0.050 0 mg/mL,respectively.The average recovery rate was 99.1%,98.6% and 98.2%,with RSD of 2.3%,1.4% and 2.0%.CONCLUSION The method is reliable,simple and accurate,which can be used to identify and evaluate the quality of Rhizoma Curcumae.

Key concepts: Chromatography, Chemistry, Calibration curve, Curcuma, High-performance liquid chromatography, Decoction, Detection limit, Traditional medicine

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