Effect of NF-κB on apoptosis of leukemia cell line induced by anti-human DR monoclonal antibody

To observe the activation of nuclear factor κB (NF-κB)and to investigate the effect of NF-κB on the apoptosis of Jurkat and U937 cells induced by anti-human DR5 monoclonal antibody (mDRA-6),total proteins of Jurkat and U937 cells was extracted ,and the activated NF-κB of Jurkat and U937 cells was detected with indirect ELISA assay. The apoptotic rate of Jurkat and U937 cells inducing by mDRA-6 were detected by MTT and flow cytometry. It was demonstrated that the level of the activated NF-κB in Jurkat and U937 cells treated with mDRA-6 for 15 minutes was significantly increased,As demonstrated by flow cytometry,the apoptotic rates of Jurkat and U937 cells acted by 10 mg/L of mDRA-6 for 4 hours were 56.63% and 34.14%respectively and the pre-treatment of these two kind of cells with 15 μmol/L of mDRA-6 inhibitor for one hour rendered the apoptotic rates of the affected cells to increase up to 76.51% and 58.01% respectively. It is concluded that anti-human DR5 monoclonal antibody can induce the apoptosis of the leukemic Jurkat and U937 cells through the expression of activated NK-κB and its inhibitor can block this effect.