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2009•Qilu Pharmaceutical AffairsRequires access

Determination of paeonol in Yangxue Tuirewan by HPLC

Lijuan Wang

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Abstract

OBJECTIVETo determine the paeonol in Yangxue Tuirewan by HPLC.METHODSUsing a column packed with octadecylsilane bonded silica gel;methanol-water(70∶30)as the mobile phase and detection wavelength was 274nm.The number of the theoretical plates of the paeonol is not less than 3500.RESULTS and CONCLUSIONThe range of paeonol was 10.2~51μg·mL-1(r=0.9999),the average recovery was 99.83%(RSD=0.47%,n=6).The method was accurate,reliable and repeatable and can be used to control the quality of Yangxue Tuirewan.

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What this paper is about

OBJECTIVETo determine the paeonol in Yangxue Tuirewan by HPLC.METHODSUsing a column packed with octadecylsilane bonded silica gel;methanol-water(70∶30)as the mobile phase and detection wavelength was 274nm.The number of the theoretical plates of the paeonol is not less than 3500.RESULTS and CONCLUSIONThe range of paeonol was 10.2~51μg·mL-1(r=0.9999),the average recovery was 99.83%(RSD=0.47%,n=6).The method was accurate,reliable and repeatable and can be used to control the quality of Yangxue Tuirewan.

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Available abstract

OBJECTIVETo determine the paeonol in Yangxue Tuirewan by HPLC.METHODSUsing a column packed with octadecylsilane bonded silica gel;methanol-water(70∶30)as the mobile phase and detection wavelength was 274nm.The number of the theoretical plates of the paeonol is not less than 3500.RESULTS and CONCLUSIONThe range of paeonol was 10.2~51μg·mL-1(r=0.9999),the average recovery was 99.83%(RSD=0.47%,n=6).The method was accurate,reliable and repeatable and can be used to control the quality of Yangxue Tuirewan.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Stationary phase, Silica gel, Methanol, Medicine

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