Determination of Paeonol in Danggui Yangxue pills by HPLC
Shen Zhang
Abstract
Shen Zhang
Abstract
Objective To establish a HPLC method for determining the paeonol content in Danggui Yangxue Pills. Methods HPLC was used to detect Paeonol in Danggui Yangxue pills with C18 column. The mobile phase consisted of methanol-H2O(70∶30). The flow rate was 1. 0 ml/min and column temperature was 25℃. The detecting wavelength was at 274 nm. Results The method was linear at the range of 0.0102~0.0510 mg/ml for Paeonol(r = 0. 9999). The average recovery rate was 99. 12%, RSD = 2.8%, n = 9). Conclusion The method is convenient and accurate, which can be applied to the content determination of paeonol and the quality control of Danggui Yangxue pills.
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Objective To establish a HPLC method for determining the paeonol content in Danggui Yangxue Pills. Methods HPLC was used to detect Paeonol in Danggui Yangxue pills with C18 column. The mobile phase consisted of methanol-H2O(70∶30). The flow rate was 1. 0 ml/min and column temperature was 25℃. The detecting wavelength was at 274 nm. Results The method was linear at the range of 0.0102~0.0510 mg/ml for Paeonol(r = 0. 9999). The average recovery rate was 99. 12%, RSD = 2.8%, n = 9). Conclusion The method is convenient and accurate, which can be applied to the content determination of paeonol and the quality control of Danggui Yangxue pills.
Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Content determination, Linear range, Pill, Chemistry, Traditional medicine