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2004•Tianjin Journal of Traditional Chinese MedicineRequires access

Determination of Paeonol in Yangyinqingfei Granula by HPLC

Jinbo Xu

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Abstract

To develop a method using HPLC for determination content of Paeonol. C18 column (4.6 mm×250 mm,10 μm) was used.Methanol-water(75∶25) was used as mobile phase. The flow rate was 1.00 mL/min. Detection was carried out at 274nm.The external standard method was used. It has a good linear correlation in 0.041 3～0.206 5 μg(r=0.999 8),the average recovery was 99.8%(RSD=1.02%,n=6).[Conclusion] This method provided simple and rapid method to analyze Paeonol in Yangyinqingfei Granula.

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What this paper is about

To develop a method using HPLC for determination content of Paeonol. C18 column (4.6 mm×250 mm,10 μm) was used.Methanol-water(75∶25) was used as mobile phase. The flow rate was 1.00 mL/min. Detection was carried out at 274nm.The external standard method was used. It has a good linear correlation in 0.041 3～0.206 5 μg(r=0.999 8),the average recovery was 99.8%(RSD=1.02%,n=6).[Conclusion] This method provided simple and rapid method to analyze Paeonol in Yangyinqingfei Granula.

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Available abstract

To develop a method using HPLC for determination content of Paeonol. C18 column (4.6 mm×250 mm,10 μm) was used.Methanol-water(75∶25) was used as mobile phase. The flow rate was 1.00 mL/min. Detection was carried out at 274nm.The external standard method was used. It has a good linear correlation in 0.041 3～0.206 5 μg(r=0.999 8),the average recovery was 99.8%(RSD=1.02%,n=6).[Conclusion] This method provided simple and rapid method to analyze Paeonol in Yangyinqingfei Granula.

Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Chemistry, Linear correlation, Mathematics, Medicine, Statistics

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