Simultaneous Determination of Chlorogenic Acid,Isofraxidin and Rosmarinic Acid in Extract of Sarcandra glabra(Thunb.) Nakai by HPLC

Objective To establish a method for simultaneous determination of chlorogenic acid,isofraxidin and rosmarinic acid in the extract of Sarcandra glabra(Thunb.)Nakai.Methods The three components were separated on a Agilent ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm)with a flow-rate of 1 mL.min-1 at 30 ℃.The mobile phase consisted of acetonitrile and 0.1 % formic acid.The gradient condition was A:5 %→20 % from 0 to 30 min,A:20 %→25 % from 30 to 40min,A:25 %→29 % from 40 to 60 min,A:29 %→32 % from 60～65min.The detection wavelength was set at 330 nm and the injection volume was 20 μL.Results The linear ranges of chlorogenic acid,isofraxidin and rosmarinic acid were 0.0280~0.448 μg(r=0.9998),0.0164~0.262 μg(r=0.9999),0.113~1.81 μg(r=0.9999),respectively.The mean recoveries(n=6) of chlorogenic acid,isofraxidin and rosmarinic acid were 103.4 %(RSD=2.77 %),98.3 %(RSD=2.58 %),95.2 %(RSD=2.49 %),respectively.Conclusion The established method is simple,reproducible,and accurate,and can be used for the simultaneous determination of chlorogenic acid,isofraxidin and rosmarinic acid in the extract of Sarcandrae glabra.