Analysis of HPLC Fingerprints and Content Determination of Isofraxidin and Rosmarinic Acid in Sarcandra glabra

OBJECTIVE:To study HPLC fingerprints of Sarcandra glabra,and to determine the contents of isofraxidin and rosmarinic acid and establish the method for quality evaluation. METHODS:The samples were separated by Inertsil ODS-4(250 mm× 4.6 mm,5 μm)column with mobile phase consisted of 0.1% phosphoric acid-acetonitrile(gradient elution)at the flow rate of 1.0 ml/ min. The detection wavelength was set at 342 nm. RESULTS:HPLC fingerprint of S. glabra was established with good separation, precision,repeatability and stability;average similarity of 10 batches of S. glabra was 0.853;there was no significant difference in fingerprint of samples from different producing areas,but the contents of 4 representative peaks were different from one another. The linear ranges of isofraxidin were 5-500 mg/ml(r=0.999 5)and rosmarinic acid were 5-500 mg/ml(r=0.998 9). Average recoveries were 105.20%(RSD=1.39%,n=6)and 99.52%(RSD=1.56%,n=6). The contents of isofraxidin and rosmarinic acid from 10 batches were obviously different from one another,especially rosmarinic acid. CONCLUSIONS:The method is simple,accurate and repeatable,which can be used for the quality control of S. glabra.