Content determination of rosmarinic acid and isofraxidin in Sarcandra glabra extract and preparations by HPLC-UVD

Objective To develop an HPLC-UVD method for content assay of rosmarinic acid and isofraxidin in Sarcandra glabra extract and preparations.Methods The method was developed on an C18 column(250 mm×4.6 mm,5 μm) by gradient elution with acetonitrile-water(both containing 0.1% formic acid)as mobile phase at a flow rate of 1.00 mL·min-1 with the wavelength at 330 nm and column temperature at 35 ℃.Results The linearity was good over the range of 4.746-189.8 mg·L-1 for rosmarinic acid and 1.018-40.72 mg·L-1 for isofraxidin.The RSD was no more than 2.0% in precision test,reproducibility test and stability test,respectively.The average recovery of rosmarinic acid and isofraxidin in Sarcandra glabra extract were 99.8%(RSD=0.70%)and 99.2%(RSD=0.63%),while in preparations were 100.7%(RSD=1.6%)and 98.4%(RSD=0.44%),respectively.The twelve batches of Sarcandra glabra extract sample shared an average content over the range of 1.479-6.447 mg·g-1 for rosmarinic acid and 1.912-3.540 mg·g-1 for isofraxidin,while 0.847-15.946 mg·g-1 for rosmarinic acid and 2.032-4.093 mg·g-1 for isofraxidin in preparations.Conclusions The method is simple,specific,precise and accurate and can help to improve the quality standard of Herba Sarcandrae extract and preparation.