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Study on Tissue Culture and Rapid Propagation Technique of Tangyujinqiu Variety of Chrysanthemum morifolium Ramat.

Zhang Li-ying

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Abstract

Tissue culture and rapid propagation technique of Tangyujinqiu variety of Chrysanthemum morifolium Ramat.with young leaves as the explants and MS as basic culture medium were studied in this paper.Results showed that the appropriate culture medium of callus induction of leaves was MS+6-BA 2mg/L+NAA 0.2mg/L;the appropriate culture medium of cluster buds induction was MS+6-BA 3mg/L+NAA 0.1mg/L;rooting medium was 1/2MS+NAA 0.1mg/L.The shoots were transplanted into the complex substrate with turf soil,vermiculite,perlite(V∶V∶V=1∶1∶1)and the survival ratio was 94%.This results provided important basis for the rapid propagation and industrial seeding culture of Tangyujinqiu.

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What this paper is about

Tissue culture and rapid propagation technique of Tangyujinqiu variety of Chrysanthemum morifolium Ramat.with young leaves as the explants and MS as basic culture medium were studied in this paper.Results showed that the appropriate culture medium of callus induction of leaves was MS+6-BA 2mg/L+NAA 0.2mg/L;the appropriate culture medium of cluster buds induction was MS+6-BA 3mg/L+NAA 0.1mg/L;rooting medium was 1/2MS+NAA 0.1mg/L.The shoots were transplanted into the complex substrate with turf soil,vermiculite,perlite(V∶V∶V=1∶1∶1)and the survival ratio was 94%.This results provided important basis for the rapid propagation and industrial seeding culture of Tangyujinqiu.

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Available abstract

Tissue culture and rapid propagation technique of Tangyujinqiu variety of Chrysanthemum morifolium Ramat.with young leaves as the explants and MS as basic culture medium were studied in this paper.Results showed that the appropriate culture medium of callus induction of leaves was MS+6-BA 2mg/L+NAA 0.2mg/L;the appropriate culture medium of cluster buds induction was MS+6-BA 3mg/L+NAA 0.1mg/L;rooting medium was 1/2MS+NAA 0.1mg/L.The shoots were transplanted into the complex substrate with turf soil,vermiculite,perlite(V∶V∶V=1∶1∶1)and the survival ratio was 94%.This results provided important basis for the rapid propagation and industrial seeding culture of Tangyujinqiu.

Key concepts: Chrysanthemum morifolium, Perlite, Tissue culture, Callus, Explant culture, Vermiculite, Botany, Horticulture

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Study on Tissue Culture and Rapid Propagation Technique of Tangyujinqiu Variety of Chrysanthemum morifolium Ramat. — Research Paper | ScholarLens