Tissue Culture and Rapid Propagation of Chrysanthemum morifolium

With the stem tips of Chrysanthemum morifoliumas the explants,the effects of different disinfection methods and culture mediums on stem tip tissue culture were studied.The results showed that the contamination rate and browning rate of the explants were the lowest by 0.1% mercuric chloride for 8min.The optimum culture medium for inducing adventitious shoots was MS+6-BA 1.0mg/L+NAA 0.05mg/L,the bud induction rate was up to 100%,and the multiple shoots grow rapidly;the optimum rooting culture medium was MS+NAA 0.5mg/L,it was easier for acclimatizing and transplanting with the sturdy root system.