Research on Tissue Culture Technology of Chrysanthemum morifolium Originated from Bozhou City

[Objective]The aim was to study the effects of different plant growth substances on callus inducing and bud differentiation of Chrysanthemum morifolium leaf.[Method]With C.morifolium leaf as explant,it was inoculated into orthogonal-Designed culture medium with two ways and was induced to form callus;then the callus was inoculated into new culture medium,the variance and range analysis were carried out on callus inducing rate and bud differentiation rate.[Result]The optimum culture medium for inducing C.morifolium leaf to form callus is MS+ 2.0 mg/L 2,4-D + 2.0 mg/L 6-BA+0.2 mg/L NAA;and the optimum culture medium for bud differentiation is MS + 2.0 mg/L 6-BA+ 0.5 mg/L NAA.[Conclusion]The study will provide high effective operation system for constructing genetic transformation system and breeding of C.morifolium.