2011•Chinese Journal of Spectroscopy LaboratoryRequires access

HPLC Fingerprint of Rose Oral Liquid for Uigur Medicine

Ajigu Abudu

Abstract

The HPLC fingerprint of rose oral liquid was established,and the quality of the product was effectively controlled.The HPLC analysis was performed on Agilent HC-C18(Analytical 4.6mm×250mm,5μm) and 0.5% phosphoric acid-methanol as mobile phase at flow rate of 0.8mL/min for gradient elution,and performance period was 40min,and column temperature was 30℃,and the sample size was 20μL,and the detection wavelength was 268nm.There were 15 common peaks in the HPLC fingerprints of rose oral liquid.This method had good repeatability.This chromatographic fingerprint method is steady,simple and reliable,and that can provide the comprehensive information for quality level of rose oral liquid.

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Key concepts: Chromatography, High-performance liquid chromatography, Repeatability, Phosphoric acid, Fingerprint (computing), Gradient elution, Chemistry, Analytical Chemistry (journal)