2009•PubMedRequires access

[Study on fingerprint of Maodongqing injection by HPLC].

Shan Lai, Liwei Yang, Guo-Deng Ou, Yongxue Li

Abstract

OBJECTIVE: To establish the fingerprint of Maodongqing injection by HPLC. METHODS: The column of ODS-2 Hypersil C18 (4.6 mm x 200 mm, 5 microm) was used. The mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution. The flow rate was 1 mL/min, the column temperature was 30 degrees C, and the detection wavelength was 325 nm. RESULTS: The fingerprint of Maodongqing injection was established, and 8 samples of different batches of Maodongqing injection were detected. Thirteen peaks in the chromatogram were marked by "Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A)". There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of the fingerprints of Chinese traditional medicine. CONCLUSION: This method is accurate, reliable and provides a scientific basis for controlling the quality of Maodongqing injection.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Chromatography, Fingerprint (computing), Phosphoric acid, Gradient elution, High-performance liquid chromatography, Chemistry, Similarity (geometry), Chromatographic separation