Effects of early intervention with Didang decoction on expression of ICAM-1 and VCAM-1 in retina of rats with diabetes

Objective To explore morphological effects and its action mechanism of intervention with Didang decoction at different time periods on retinopathy in diabetic rats through streptozotocin(STZ)-induced diabetic rat model.Methods With caudal vein injection of STZ and high fat diet to make diabetic rat model,all the rats were divided into a normal control group and a model group,and the model group was divided into a model control group,a late stage of Didang decoction group,a middle stage of Didang decoction group,an early stage of Didang decoction group,a simvastatin group and a rosiglitazone maleate group.The effects of Didang decoction on diabetic rats’weight,blood glucose and insulin,as well as,the morphological change in retina after HE staining and expression of intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) by immumohistochemical staining were observed.Results At the 13th week of the experiment,compared with the normal control group,fasting blood glucose in the model group was obviously increased with statistical significance(P 0.01);Compared with the model control group,the rats in the early stage of Didang decoction group had relatively mild retinopathy of HE staining.Compared with the model control group,the expression of ICAM-1 and VCAM-1 of immumohistochemical staining in the rats’retina was decreased in the early stage of Didang decoction group(P 0.01).Conclusion The early intervention with Didang decoction could delay the development of retinopathy in diabetic rats.