Effect of Didang Decoction Medicated Early to Type 2 Diabetic Rats on Serum IL-4 and IL-13 Level and Aorta TNF-αmRNA Expression

Objective:To observe the protective effects of early intervention with Didang Decoction on the macrovascular lesions of the experimental type 2 diabetic rats.Methods:The diabetic rat models were established after being injected streptozotocin(STZ) into the caudal vein and had fed with high fat diet for 12 weeks.The rats in Didang decoction late group,Didang decoction mid-term group and Didang decoction early group were given Didang decoction from the 16th,13th and 8th week respectively to the 24th week.The rats in simvastatin group and rosiglitazone group were given simvastatin and rosiglitazone respectively from the 13th to 24th week respectively.Then detecting the level of IL-4 and IL-13 in the serum using ELASA method and the mRNA expression of TNF-α in the aorta using Real-time PCR method.Results:Compared with normal control group,the level of IL-4vand IL-13vin the serum was lower and the expression of TNF-αmRNA in the aorta was higher in model control group,and P0.05 respectively;compared with model control group,the level of IL-4vin the serum was higher in Didang Decoction early group and simvastatin group(P0.05),and the level of IL-13 in the serum was higher in Didang Decoction early group,Didang Decoction mid-term group and rosiglitazone group(P0.05);compared with normal control group,the expression of TNF-αmRNA in the aorta was higher in model control group(P0.05);compared with model control group,the expression of TNF-αmRNA in the aorta was lower in Didang Decoction Early Group,Didang Decoction mid-term group and rosiglitazone group(P0.05).Conclusion:Giving Didang Decoction early to the experimental type 2 diabetic rats can raise the level of IL-4 and IL-13 in the serum,and can reduce the mRNA expression of TNF-α in the aorta.Thus the inflammatory injury of macrovascular disease is inhibited,and the development of diabetic vascular disease is delayed.