Effects of early intervention with Didang decoction on expression of VCAM-1 and TNF-α in experimental type 2 diabetic rats.

Objective To observe the protective effects of the early intervention with Didang decoction on the experimental type 2 diabetic rats with macrovascular lesions.Methods Experimental diabetic rats were induced by high fat diet and streptozotocin.The rats in Didang decoction late group,Didang decoction middle group and Didang decoction early group were given Didang decoction from 4 weeks before modeling,modeling completed and 4 weeks after modeling respectively to 24th week.The rats in simvastatin group and rosiglitazone group were given simvastatin and rosiglitazone respectively from modeling completed to 24th week.The level of VCAM-1 in serum was detected by ELASA and the mRNA expression of TNF-α in aorta using real-time PCR.Results Compared with normal control group,the level of VCAM-1 in serum and the mRNA expression of TNF-α in aorta were higher in model control group(P0.05).Compared with model control group,the level of VCAM-1 in serum was lower in Didang decoction early group and simvastatin group(P0.05),the mRNA expression of TNF-α in aorta was lower in Didang decoction early group,Didang decoction middle group and rosiglitazone group(P0.05).Conclusion The level of VCAM-1 in serum was reduced and the mRNA expression of TNF-α in aorta of experimental type 2 diabetic rats was inhibited after early intervened with Didang decoction.Thus the inflammatory injury of macrovascular lesions was inhibited,the development of diabetic vascular disease was delayed,and the great vessels was protected.