The Effects of E-selectin Gene A128C Polymorphisms on Coronary Heart Disease

Objective:To explore whether E-selectin gene single-base A/C polymorphism in exon 3 codon 128 related to coronary heart disease (CHD) in Chinese Han peoples. To study the correlation between the genotype and its serum soluble E-selectin concentratioa Methods:The genotypes of E-selectin were detected by polymerase chain reaction-restriction fragment length polymorphism methods in 145 CHD including 73 Acute Coronary Syndrom (ACS) patients and 72 stable CHD patients and 144 healthy controls. The serum concentrations of E-selectin were measured by enzyme-linked immunosorbent assay. Results:There was significant difference in frequencies of genotype and allele in A128C polymorphism between CHD and control group as well as between ACS and stable CHD respectively(P0.001 and P0.05). The relative risk suffer from CHD with genotype CC+AC was 5. 843 times of those with genotype AA (OR=5.843,95%CI:3.066-11.133). The serum E-selectin concentrations of genotype CC+AC were significantly higher than those of genotype AA (49.9±9.6ng/ml vs. 35.3±7.4ng/ ml, P0. 01). Conclusion: E-selectin gene A128C polymorphism is significantly associated with the severity of CHD. Its polymorphyism may affect the concentrations of serum E-selectin. C allele of E-selectin may be a genetic factor that may determine an individual's susceptibility for CHD.