The serum E-selectin level and the G98T,S128R polymorphisms of E-selectin in patients with coronary heart disease

Objective To study the association between the G98T polymorphism in the exon 2 and the S128R polymorphism in the exon 4 of E selectin gene and coronary heart disease (CHD) in Chinese Han peoples, and to evaluate the effect of E selectin gene polymorphisms on the serum E selectin level in patients with coronary heart disease Methods The genotypes of E selectin were detected by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) methods in 238 CHD patients and 199 healthy controls.The serum level of E selectin was determined by enzyme linked immunosorbent assay(ELISA). Results There was significant difference in frequencies of allele and genotype in S128R polymorphism between CHD and control groups respectively. The relative risk suffered from CHD with SR genotype was 2 162 times of those with SS genotype( OR =2 162,95% CI :1 073～4 359), the serum E selectin level was significantly higher among carriers of SR genotype as compared with non carriers(SS genotype) (42 9±8 1 vs 35 7±7 7, P 0 01) There was no significant difference in genotype distribution for the E selectin gene G98T polymorphism between CHD and control groups( P 0 05), but the distribution of allele and genotype in E selectin G98T was of significant difference between myocardial infarction and angina pectoris groups( P 0 05). Conclusions E selectin S128R polymorphism is associated with CHD, its polymorphism may affect the serum E selectin level, and R allele may be a risk factor for CHD. The polymorphism of E selectin gene G98T may not play an important and direct role in the pathogenesis of CHD