Influence of Ziyin Yiqi Huoxue Jiedu Fang on SREBP-1c expression in mice with diabetes complicating fatty liver

Objective To investigate the influence of Ziyin Yiqi Huoxue Jiedu Fang on SREBP-1c expression in transgenic MKR mice with type 2 diabetes complicating fatty liver induced by high fat diet. Methods MKR mice(n=30) were randomly divided into control group,model group and Ziyin Yiqi Huoxue Jiedu(Ziyin group).The model group and Ziyin group were fed with high fat feeds and control group with basic feeds for 8 weeks.After 8 weeks Ziyin group was intragastrically given Chinese medicinal(crude medicinal,29.64 g/kg) for 4 weeks.Other two groups were given distilled water.The hepatic steatosis was observed by using light microscope.The expression of SREBP-1 c mRNA was detected by using reverse transcription polymerase chain reaction(RT-PCR) and expression of SREBP-1 c protein was detected by using immunohistochemistry technique.Results The expressions of SREBP-1 c mRNA and SREBP-1 c protein increased in model group compared with control group(P0.01).The expressions of SREBP-1 c mRNA and SREBP-1 c protein decreased in Ziyin group,and difference in theexpression of SREBP-1c mRNA had statistical significance between Ziyin group and model group(P0.01).Conclusion Ziyin Yiqi Huoxue Jiedu Fang can relieve the hepatic steatosis through regulating the expression of SREBP-1 c in mice with diabetes complicating fatty liver.