Effect of Ziyin Huoxue Jiedu Decoction on the Contents of Prothrombin mRNA and PAR-1 mRNA of Focal Cerebral Infarction Rats

Objective To observe the effect of Ziyin Huoxue Jiedu decoction on the expression of prothrombim mRNA and protease-activated receptor-1(PAR-1) mRNA of focal cerebral infarction rats.Methods Twenty-four rats were randomly divided into 4 groups:the sham-operated group,ischemic group,Ziyin Huoxue Jiedu decoction group,Argatroban group.Each group was divided into two time phase point of 1,3 d.Ziyin Huoxue Jiedu decoction group was fed with Chinese medicine decoction with the daily dose of 32.4 g/kg.Argatroban group was given the intraperitoneal injection,with the daily dose of 1,2 d 11.6 mg/kg,day 3 dose of 3.72 mg/kg.The sham-operated group and ischemic group were fed with saline with the daily dose of 2 mL/100 g.Daily dose divided equally into 2 times.The model was reproduced by occluded middle cerebral artery.The expression of prothrombin mRNA and PAR-1 mRNA of brain tissue were detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The expression of prothrombin mRNA and PAR-1 mRNA of brain tissue in model group was significantly higher than that in the sham-operated group(P0.01),and the expression of prothrombin mRNA and PAR-1mRNA of brain tissue in Ziyin Huoxue Jiedu decoction group and Argatroban group was significantly lower than that in the model group(P0.01).Conclusion Ziyin Huoxue Jiedu decoction is effective to restrain the expression of prothrombin mRNA and PAR-1 mRNA of brain tissue,reducing thrombin toxic injury,which may be one of the mechanisms of cerebral protective effects of Ziyin Huoxue Jiedu decoction.