Surveillance and analysis on antimicrobial resistance of bacteria isolated from a hospital from 2005 to 2007

Objective To investigate antimicrobial resistance of clinical isolates obtained from a hospital and provide guidance for rational use of antimicrobial agents.Methods Clinical isolated bacterial strains were collected from a hospital from 2005 to 2007,baterial identification and routine biochemical tests were performed with VITEK system,antimicrobial susceptibility tests of isolates were carried out by Kirby-Bauer method.The data were analyzed with the software of WHONET 5.1.Results A total of 4 014 pathogenic strains were collected,1 551(38.64%) and 2 463(61.36%) of which were gram-positive cocci and gram-negative bacilli respectively.The constitutional ratio of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase negative staphylococcus was 49.82% and 82.16% respectively;vancomycin-resistant gram-positive cocci were not found.The detection rate of extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumonia isolates was 50.95% and 47.53% respectively.Among nonfermentative gram-negative bacilli,the resistant rates of Pseudomonas aeruginosa to imipenem and meropenem was 30.20% and 27.06% respectively,and 19.62% and 22.15% for Acinetobacter baumannii respectively.Stenotrophomonas maltophilia was sensitive to trimethoprim/sulfamethoxazole and levofloxacin,drug-resistant rate was 6.85% and 9.59% respectively.Conclusion Regular surveillance of antimicrobial resistance in clinical isolates is important for us to realize the trend of antimicrobial resistance and rational antimicrobial therapy.